Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis
Identifieur interne : 001237 ( Main/Exploration ); précédent : 001236; suivant : 001238Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis
Auteurs : Lei Zhang [République populaire de Chine] ; Yan Chen [République populaire de Chine] ; Lin Wang [République populaire de Chine] ; Xiao-Ping Chen [République populaire de Chine] ; Wan-Guang Zhang [République populaire de Chine] ; Chun-You Wang [République populaire de Chine] ; He-Shui Wu [République populaire de Chine]Source :
- Journal of Huazhong University of Science and Technology [Medical Sciences] [ 1672-0733 ] ; 2013-06-01.
Descripteurs français
- KwdFr :
- Animaux, Chloroquine (usage thérapeutique), Cytokines (immunologie), Lésion pulmonaire aigüe (anatomopathologie), Lésion pulmonaire aigüe (immunologie), Lésion pulmonaire aigüe (traitement médicamenteux), Mâle, Pancréatite aigüe nécrotique (), Pancréatite aigüe nécrotique (anatomopathologie), Rat Wistar, Rats, Récepteur de type Toll-4 (immunologie), Résultat thérapeutique.
- MESH :
- anatomopathologie : Lésion pulmonaire aigüe, Pancréatite aigüe nécrotique.
- immunologie : Cytokines, Lésion pulmonaire aigüe, Récepteur de type Toll-4.
- traitement médicamenteux : Lésion pulmonaire aigüe.
- usage thérapeutique : Chloroquine.
- Animaux, Mâle, Pancréatite aigüe nécrotique, Rat Wistar, Rats, Résultat thérapeutique.
English descriptors
- KwdEn :
- Acute Lung Injury (drug therapy), Acute Lung Injury (immunology), Acute Lung Injury (pathology), Animals, Chloroquine (therapeutic use), Cytokines (immunology), L -arginine, Male, N-nitro- L -arginine methyl ester, Pancreatitis, Acute Necrotizing (complications), Pancreatitis, Acute Necrotizing (pathology), Rats, Rats, Wistar, Toll-Like Receptor 4 (immunology), Toll-like receptor, Treatment Outcome, acute hemorrhagic necrotizing pancreatitis, chloroquine, lung, nitric oxide.
- MESH :
- chemical , immunology : Cytokines, Toll-Like Receptor 4.
- chemical , therapeutic use : Chloroquine.
- complications : Pancreatitis, Acute Necrotizing.
- drug therapy : Acute Lung Injury.
- immunology : Acute Lung Injury.
- pathology : Acute Lung Injury, Pancreatitis, Acute Necrotizing.
- Animals, Male, Rats, Rats, Wistar, Treatment Outcome.
Abstract
Summary: This study preliminarily investigated the mechanism by which chloroquine (CQ) relieves acute lung injury (ALI) complicated in acute hemorrhagic necrotizing pancreatitis (AHNP). Sixty male Wistar rats were randomized into sham-operated group (group A, n=10), AHNP group (group B, n=10), L-arginine-treated group (group C, n=10), L-N-nitro-L-arginine methyl ester (NAME)-treated group (group D, n=10), CQ-treated group (group E, n=10) and CQ+L-NAME-treated group (group F, n=10). TLR4 expression was measured by using real time-PCR and Western blotting respectively. The results showed that, in the group B, the expression of TLR4 and the levels of TNF-α and IL-6 in the lungs were significantly increased, and the nitric oxide (NO) concentration was reduced, as compared with those in the group A (P<0.05 or P<0.01). Lung injury was aggravated with the increased expression of TLR4. When the inhibitor and stimulator of TLR4, namely L-Arg and L-NAME, were added respectively, lung injury was correspondingly relieved or aggravated (P<0.05 or P<0.01). In the group E, TLR4 expression was substantially lower and NO concentration higher than those in the group B (P<0.05 or P<0.01). However, in the group F, NO concentration was markedly decreased, and the inhibitory effect of CQ on TLR4 expression and the relief of lung injury were weakened when compared with those in the group E (P<0.05 or P<0.01). It was concluded that TLR4 may play an important role in the pathogenesis and development of ALI complicated in AHNP. CQ could relieve ALI by decreasing the TLR4 expression and increasing the NO release.
Url:
DOI: 10.1007/s11596-013-1124-9
Affiliations:
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Huazhong Univ. Sci. Technol. [Med. 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Sixty male Wistar rats were randomized into sham-operated group (group A, n=10), AHNP group (group B, n=10), L-arginine-treated group (group C, n=10), L-N-nitro-L-arginine methyl ester (NAME)-treated group (group D, n=10), CQ-treated group (group E, n=10) and CQ+L-NAME-treated group (group F, n=10). TLR4 expression was measured by using real time-PCR and Western blotting respectively. The results showed that, in the group B, the expression of TLR4 and the levels of TNF-α and IL-6 in the lungs were significantly increased, and the nitric oxide (NO) concentration was reduced, as compared with those in the group A (P<0.05 or P<0.01). Lung injury was aggravated with the increased expression of TLR4. When the inhibitor and stimulator of TLR4, namely L-Arg and L-NAME, were added respectively, lung injury was correspondingly relieved or aggravated (P<0.05 or P<0.01). In the group E, TLR4 expression was substantially lower and NO concentration higher than those in the group B (P<0.05 or P<0.01). However, in the group F, NO concentration was markedly decreased, and the inhibitory effect of CQ on TLR4 expression and the relief of lung injury were weakened when compared with those in the group E (P<0.05 or P<0.01). It was concluded that TLR4 may play an important role in the pathogenesis and development of ALI complicated in AHNP. CQ could relieve ALI by decreasing the TLR4 expression and increasing the NO release.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Zhang, Lei" sort="Zhang, Lei" uniqKey="Zhang L" first="Lei" last="Zhang">Lei Zhang</name></region><name sortKey="Chen, Xiao Ping" sort="Chen, Xiao Ping" uniqKey="Chen X" first="Xiao-Ping" last="Chen">Xiao-Ping Chen</name><name sortKey="Chen, Xiao Ping" sort="Chen, Xiao Ping" uniqKey="Chen X" first="Xiao-Ping" last="Chen">Xiao-Ping Chen</name><name sortKey="Chen, Yan" sort="Chen, Yan" uniqKey="Chen Y" first="Yan" last="Chen">Yan Chen</name><name sortKey="Wang, Chun You" sort="Wang, Chun You" uniqKey="Wang C" first="Chun-You" last="Wang">Chun-You Wang</name><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name><name sortKey="Wu, He Shui" sort="Wu, He Shui" uniqKey="Wu H" first="He-Shui" last="Wu">He-Shui Wu</name><name sortKey="Wu, He Shui" sort="Wu, He Shui" uniqKey="Wu H" first="He-Shui" last="Wu">He-Shui Wu</name><name sortKey="Zhang, Lei" sort="Zhang, Lei" uniqKey="Zhang L" first="Lei" last="Zhang">Lei Zhang</name><name sortKey="Zhang, Wan Guang" sort="Zhang, Wan Guang" uniqKey="Zhang W" first="Wan-Guang" last="Zhang">Wan-Guang Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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